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MELK Antibody (C-term)
Catalog Number: orb1929525
| Catalog Number | orb1929525 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525-554 amino acids from the C-terminal region of human MELK. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | Q14680 |
| MW | 74642 Da |
| Tested applications | WB |
| Dilution range | WB: 1:1000, WB: 1:1000 |
| Antibody Type | Primary Antibody |
| Clone Number | RB8557 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Maternal embryonic leucine zipper kinase, hMELK, P Read more... |
| Note | For research use only |
| NCBI | NP_001243614.1, NP_001243621.1, NP_001243619.1, NP_001243622.1, NP_001243617.1, NP_055606.1, NP_001243616.1, NP_001243618.1 |
The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate
All lanes: Anti-MELK Antibody C-term at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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