MELK Antibody (C-term)

SKU: orb1929525

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD0779
• Immunogen: This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525-554 amino acids from the C-terminal region of human MELK. Antigen Region: 525-554 aa.
• Target: MELK
• Molecular Weight: 74642 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK, MELK, KIAA0175

The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate

All lanes: Anti-MELK Antibody C-term at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

MELK

UniProt

Q14680

RefSeq (Protein):NP_001243614.1, NP_001243619.1, NP_001243617.1, NP_001243621.1, NP_001243622.1, NP_001243616.1, NP_001243618.1, NP_055606.1