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(Mouse) Melk Antibody (C-term)

Catalog Number: orb1926547

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1926547
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB51204
Tested applicationsFC, WB
ReactivityMouse
IsotypeRabbit IgG
Dilution rangeWB: 1:1000, WB: 1:2000, WB: 1:1000, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW72729 Da
TargetThis mouse Melk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 434-468 amino acids from the C-terminal region of mouse Melk.
UniProt IDQ61846
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesMaternal embryonic leucine zipper kinase, Protein
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NoteFor research use only
Expiration Date12 months from date of receipt.
(Mouse) Melk Antibody (C-term)

Anti-Melk Antibody (C-term) at 1:1000 dilution + mouse ovary lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

(Mouse) Melk Antibody (C-term)

Anti-Melk Antibody (C-term) at 1:1000 dilution + mouse testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

(Mouse) Melk Antibody (C-term)

Anti-Melk Antibody (C-term) at 1:2000 dilution + mouse spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

(Mouse) Melk Antibody (C-term)

Overlay histogram showing MCF-7 cells stained (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

  • (Mouse) Melk Antibody (C-term) [orb1167230]

    FC,  WB

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl