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Melanoma Marker (MART-1 + gp100) Antibody
Catalog Number: orb607976
| Catalog Number | orb607976 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to Melanoma Marker (MART-1 + gp100) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | DT101+ BC199 + HMB45 |
| Tested applications | IHC |
| Reactivity | Human |
| Isotype | IgG1|IgG2b |
| Immunogen | Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
| Concentration | Purified Ab with BSA and Azide at 200ug/ml |
| Dilution range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Conjugation | Unconjugated |
| MW | 20-22kDa (doublet) (MART-1): 90-100kDa (gp100) |
| Target | MLANA |
| Entrez | 2315, 6490 |
| UniProt ID | Q16655, P40967 |
| Expression System | Bioreactor |
| Storage | Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Alternative names | Melanoma antigen recognized by T-cells 1 (MART-1), Read more... |
| Note | For research use only |
| Application notes | Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues |
| Expiration Date | 12 months from date of receipt. |
