Melanoma Marker Antibody Cocktail (MART-1 & Tyrosinase & gp100)

• Catalog Number: orb640123
• Category: Antibodies
• Description: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DT101+ BC199 + T311 + HMB45
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa + Mouse IgG1, kappa + Mouse IgG2a, kappa
• Immunogen: Recombinant hMART-1 protein (DT101 & BC199), extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant tyrosinase protein (T311) were used as the immunogen for this Melanoma Marker antibody cocktail.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Melanoma Marker antibody cocktail is available for research use only.
• Storage: Store the Melanoma Marker antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.