Melanoma Marker Antibody Cocktail (MART-1 & Tyrosinase & gp100)

SKU: orb2644193

Description

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Research Area

Cancer Biology; Cell Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Melanoma Marker antibody cocktail should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, IgG2a, IgG2b / kappa, lambda
• Clone No.: DT101+ BC199 + T311 + HMB45
• Immunogen: Recombinant hMART-1 protein (DT101 & BC199), extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant tyrosinase protein (T311) were used as the immunogen for this Melanoma Marker antibody cocktail.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + T311 + HMB45). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.