Melan-A Antibody [SPM555]

• Catalog Number: orb1251845
• Category: Antibodies
• Description: Melan-A Antibody [SPM555]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM555
• Tested applications: FC, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for this Melan-A antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MLANA
• UniProt ID: Q16655
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the Melan-A antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (SPM555).