MLANA Antibody

SKU: orb1252767

Description

MLANA Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 0.5-1ug/500ug protein lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: M2-9E3
• Immunogen: Recombinant human Melan-A protein was used as the immunogen for this antibody.
• Target: MLANA
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.

Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.

SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone M2-9E3). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

MLANA

UniProt

Q16655