MEK2 N-Term Antibody

SKU: orb420326

Description

MEK2 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:40,000, IHC: 1:100, WB: 1µg/mL
• Reactivity: Human, Mouse
• Application Notes:
  Anti-MEK 2 (RABBIT) antibody has been tested in ELISA, Western Blotting, and IHC. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
• Purity: This affinity purified antibody is directed against human MEK2 protein. Anti-MEK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MEK2 antibody, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2, ERK activator kinase 2, MAPK/ERK kinase 2

Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody orb420326 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

Western Blot of Anti-MEK2 N-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Lane 3: Mouse Brain Whole cell lysate (p/n orb348715). Lane 4: HEK293T Whole cell lysate. Load: 10 ug. Primary Antibody: Anti-MEK2 at 1 µg/ml overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody at 1:40000 for 30 min at RT. Blocking: BlockOut Universal Blocking buffer orb348644. Predicted MW: 45kDa.

Quick Database Links

UniProt

P36507