MEK2 (MAP2K2) Antibody (Center)

• Catalog Number: orb1928729
• Category: Antibodies
• Description: MEK2 (MAP2K2) Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P36507
• MW: 44424 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: MEK2, MKK2, PRKMK2
• Research Area: Cancer Biology, Immunology & Inflammation, Immunol
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-MAP2K2 Pab is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.

MAP2K2 Antibody (A278) western blot analysis in U937 cell line lysates (35 ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).

Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.