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MAP2K2 Antibody

SKU: orb420338

Description

MEK2 antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 5.0 µg/ml, IHC: 1:100, WB: 1.0 ug/mL
ReactivityHuman
Application Notes
Anti-MEK 2 (RABBIT) antibody has been tested in ELISA, Western Blotting, and IHC. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus human MEK2 protein conjugated to KLH.
TargetMAP2K2
PurityThis affinity purified antibody is directed against human MEK2 protein. Anti-MEK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.09 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-MEK2 antibody, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2, ERK activator kinase 2, MAPK/ERK kinase 2

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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25 μl
Free Secondary Antibody (20 ul)0/0

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