MEK1 (MAP2K1) Antibody (S218)

SKU: orb1928733

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human
• Predicted Reactivity: Drosophila, Hamster, Mouse, Other, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MEK1(MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from human MEK1(MAP2K1). Antigen Region: 196-225 aa.
• Target: MAP2K1 (HGNC:6840)
• Molecular Weight: 43439 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1

Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1 Antibody (S217).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2).

All lanes: Anti-MAP2K1 Antibody at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

MAP2K1 (HGNC:6840)

UniProt

Q02750

RefSeq (Protein):NP_002746.1