MECL1 Antibody

• Catalog Number: orb1249607
• Category: Antibodies
• Description: MECL1 Antibody
• Target: PSMB10
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-PTEPVKRSGRYH
• UniProt ID: P40306
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9kDa according to NP_002792.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows efined staining in a subset of cells. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PSMB10, proteasome (prosome, macropain) subunit, b
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: NP_002792.1

orb1249607 (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249607 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.