PSMB10 Antibody

SKU: orb1257484

Description

PSMB10 Antibody

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
• Target: PSMB10
• Molecular Weight: Observed: 29kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i

Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (orb1257484) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (orb1257484) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (orb1257484) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using PSMB10 antibody (orb1257484). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

PSMB10

UniProt

P40306