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PSMB10 Antibody
SKU: orb1257484
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1). |
| Target | PSMB10 |
| Molecular Weight | Observed: 29kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i
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Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (orb1257484) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (orb1257484) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (orb1257484) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using PSMB10 antibody (orb1257484). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
PSMB10
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
PSMB10 Antibody (orb1257484)
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