ME2 Antibody (C-term)

• Catalog Number: orb1925547
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 970CT2.1.1.2
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG1,k
• Dilution range: WB: 1:4000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 65444 Da
• Target: This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.
• UniProt ID: P23368
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NAD-dependent malic enzyme, mitochondrial, NAD-ME,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-ME2 Antibody (C-term) at 1:4000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.