ME2 Antibody

SKU: orb1473945

Description

The ME2 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ME2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1/1000 - 1/2000), IH (1/50 - 1/200)
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ME2. The exact sequence is proprietary.
• Purification: This antibody is purified through a protein G column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

NAD-dependent malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2

Western blot analysis of ME2 expression in HL60 (A), NIH3T3 (B), Jurkat (C) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 65 kD)

Immunohistochemical analysis of ME2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P23368, Q99KE1

NCBI Gene ID

107029, 4200