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Catalog Number | orb11034 |
---|---|
Category | Antibodies |
Description | MDR1/P Glycoprotein Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human MDR1 (1051-1280/1280aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, ICC/IF=1:100, Flow-Cyt=1μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 141 kDa |
Target | ABCB1 |
UniProt ID | P08183 |
RRID | AB_10749060 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | ABC20; ABCB1; ATP binding cassette, sub family B(M Read more... |
Background | _CLIENT_OVERRIDE_ |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (orb11034), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at -20°C) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: liver (Rat) Lysate at 40 ug, Primary: Anti-MDR1'P Glycoprotein (orb11034) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 141kD, Observed band size: 141 kD.
Tissue/Cell: Rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MDR1 / P Glycoprotein Polyclonal Antibody, Unconjugated (orb11034) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (orb11034) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (orb11034) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
FC, ICC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |
FC, ICC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE/Cy5 |
FC, ICC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |
FC, ICC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Cy5.5 |
FC, ICC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Cy7 |