MDR1 Rabbit Polyclonal Antibody

• Catalog Number: orb221459
• Category: Antibodies
• Description: MDR1 Rabbit Polyclonal Antibody
• Target: ABCB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human MDR1 (21-100/1272aa)
• UniProt ID: P08183
• MW: 141 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: P-Glycoprotein; Multi Drug Resistance Associated P
• Note: For research use only

Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20°C) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (orb221459), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-MDR1 (orb221459) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 150-180 kD, Observed band size: 150 kD.

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-MDR1 (orb221459) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 141kD, Observed band size: 150kD.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (orb221459) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (orb221459) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.