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Catalog Number | orb221459 |
---|---|
Category | Antibodies |
Description | MDR1 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human MDR1 (21-100/1272aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 141 kDa |
Target | ABCB1 |
UniProt ID | P08183 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | P-Glycoprotein; Multi Drug Resistance Associated P Read more... |
Background | _CLIENT_OVERRIDE_ |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20°C) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (orb221459), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-MDR1 (orb221459) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 150-180 kD, Observed band size: 150 kD.
Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-MDR1 (orb221459) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 141kD, Observed band size: 150kD.
Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (orb221459) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (orb221459) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |