MC5R Antibody

• Catalog Number: orb1249593
• Category: Antibodies
• Description: MC5R Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: PNVKNKSSPCED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptideImmunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MC5R
• UniProt ID: P33032
• NCBI: NP_005904.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MC5R N: melanocortin 5 receptor
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptideImmunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249593 (0.3 ug/ml) staining of Mouse Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249593 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.