MC5R Antibody

• Catalog Number: orb1249593
• Category: Antibodies
• Description: MC5R Antibody
• Target: MC5R
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: PNVKNKSSPCED
• UniProt ID: P33032
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptideImmunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MC5R N: melanocortin 5 receptor
• Note: For research use only
• NCBI: NP_005904.1

orb1249593 (0.3 ug/ml) staining of Mouse Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249593 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.