Goat anti-MC5R Antibody

• Catalog Number: orb20150
• Category: Antibodies
• Description: Goat polyclonal antibody to MC5R
• Target: MC5R
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PNVKNKSSPCED
• RRID: AB_10750617
• MW: 36.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 37kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MC5R N: melanocortin 5 receptor antibody
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 4161

orb20150 (0.3ug/ml) staining of Mouse Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Cortex using MC5R antibody