Maspin Antibody

• Catalog Number: orb1272660
• Category: Antibodies
• Description: Maspin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Human Maspin specific antibody was purified by affinity chromatography employing an immobilized Human Maspin matrix.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: SERPINB5
• UniProt ID: P36952
• NCBI: P36952
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PI5, maspin, PI5, Serpin B5, Maspin, PI-5
• Note: For research use only
• Application notes: ELISA:In a sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Maspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Western Blot:To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (orb1272629) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.

To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.