MAPKAPK2 Antibody

SKU: orb345385

Description

MAPKAP Kinase 2 antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:15,000, WB: 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-MAPKAP kinase 2 antibody is tested by western blotting and suitable for ELISA, immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated (p/n w09-000-364) or TNFα stimulated ((p/n w09-000-367) human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
• Target: MAPKAPK2
• Purity: This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish. Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.9 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MAPKAP kinase 2 antibody, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase-2, MK2, mitogen-activated protein kinase-activated protein kinase-2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2

Quick Database Links

Gene Symbol

MAPKAPK2

UniProt

P49137

RefSeq:P49137.1