MK2/MAPKAPK2 Antibody

• Catalog Number: orb107653
• Category: Antibodies
• Description: Goat polyclonal to MK2
• Target: MK2 / MAPKAPK2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SRVLKEDKERWED
• MW: 42.2; 45.6
• Tested applications: ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:128000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MK2 antibody
• Note: For research use only
• Entrez: 9261

Western blot analysis MEF lysates (35ug protein in RIPA buffer) using MK2 antibody

Western blot analysis of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in paral

Western blot analysis of HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed using MK2 antibody