MAPK14 Antibody (Y323)

SKU: orb1929462

Description

MAPK14 Antibody (Y323)

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: FC - 1:25, WB - 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 41293 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

MAPK14 Antibody (Y322) western blot analysis in Jurkat cell line and mouse kidney tissue lysates (35 ug/lane). This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).

All lanes: Anti-MAPK14 Antibody (Y323) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MAPK14 Antibody (Y323) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: PC-12 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q16539