MAP4K3 Antibody

• Catalog Number: orb1939368
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 219CT8.3.1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgM,k
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:500~1000, IHC-P: 1:100
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 101316 Da
• Target: This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein.
• UniProt ID: Q8IVH8
• NCBI: NP_003609.2, NP_001257354.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase kinase kinase kin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MAP4K3 western blot analysis in A2058 cell line lysates (35 μg/lane). This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).

Western blot analysis of lysate from MCF-7 cell line, using MAP4K3. diluted at 1:1000. A goat anti-mouse IgM H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysate at 20 μg.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MAP4K3 Antibody. diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from HepG2, MCF-7 cell line (from left to right), using MAP4K3 Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgM H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 35 μg per lane.