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MAML3 Antibody

Catalog Number: orb750174

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750174
CategoryAntibodies
DescriptionMastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jkappa; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.
Species/HostMouse
ClonalityMonoclonal
Clone NumberMMLP3-1
Tested applicationsIHC-P
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA human full length recombinant protein was used as the immunogen for this MAML3 antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml (1)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis MAML3 antibody is available for research use only.
Entrez55534
StorageStore the MAML3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
Expiration Date12 months from date of receipt.
MAML3 Antibody

IHC testing of FFPE human placenta with MAML3 antibody (clone MMLP3-1).

MAML3 Antibody

IHC testing of FFPE human colon carcinoma with MAML3 antibody (clone MMLP3-1).

MAML3 Antibody

IHC testing of FFPE human cervical carcinoma with MAML3 antibody (clone MMLP3-1).

MAML3 Antibody

IHC testing of FFPE human pancreas with MAML3 antibody (clone MMLP3-1).

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