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MAML3 Antibody

Catalog Number: orb606362

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606362
CategoryAntibodies
DescriptionMastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenA human full length recombinant protein was used as the immunogen for this MAML3 antibody.
Tested applicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.
Antibody TypePrimary Antibody
Clone NumberMAML3/1303
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis MAML3 antibody is available for research use only.
NoteFor research use only
MAML3 Antibody

IHC testing of FFPE human pancreas with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

MAML3 Antibody

IHC testing of FFPE human cervical carcinoma with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

MAML3 Antibody

IHC testing of FFPE human colon carcinoma with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

MAML3 Antibody

IHC testing of FFPE human placenta with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

MAML3 Antibody

SDS-PAGE Analysis of Purified, BSA-Free MAML3 Antibody (clone MAML3/1303). Confirmation of Integrity and Purity of the Antibody.

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    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
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    FC,  IF,  IHC

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    Monoclonal

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    FC,  IF,  IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Anti-MAML3 Antibody [orb1461088]

    FC,  IF,  IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • MAML3 Antibody [orb750174]

    IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg