MAML3 Antibody

• Catalog Number: orb606362
• Category: Antibodies
• Description: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MAML3/1303
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MAML3 antibody is available for research use only.
• Entrez: 55534
• Storage: Store the MAML3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human cervical carcinoma with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human placenta with MAML3 antibody (clone MAML3/1303). Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free MAML3 Antibody (clone MAML3/1303). Confirmation of Integrity and Purity of the Antibody.