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Catalog Number | orb1239608 |
---|---|
Category | Antibodies |
Description | MAGEA4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.The immunogen is located within the first 50 amino acids of MAGEA4. |
Concentration | 1 mg/mL |
Dilution range | MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 35 kDa Observed: 45 kDa |
Target | MAGEA4 |
UniProt ID | P43358 |
NCBI | NP_001011550 |
Storage | MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. |
Buffer/Preservatives | MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A Read more... |
Note | For research use only |
Application notes | MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 µg/ml.
Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 µg/mL.
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 µg/ml.
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 µg/ml. Green: MAGEA4 Antibody (orb1239608) Blue: DAPI staining.
Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 µg/ml.
Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 µg/ml. Red: MAGEA4 Antibody (orb1239608) Blue: DAPI staining.
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