MAGEA4 Antibody

SKU: orb1239608

Description

MAGEA4 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.The immunogen is located within the first 50 amino acids of MAGEA4.
• Target: MAGEA4
• Molecular Weight: Predicted: 35 kDa Observed: 45 kDa
• Purification: MAGEA4 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A, MAGE-4 antigen, MAGE4B, MAGE-X2, MAGE-X2 antigen, Melanoma-associated antigen 4

Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 µg/ml.

Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 µg/mL.

Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 µg/ml.

Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 µg/ml. Green: MAGEA4 Antibody (orb1239608) Blue: DAPI staining.

Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 µg/ml.

Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 µg/ml. Red: MAGEA4 Antibody (orb1239608) Blue: DAPI staining.

Quick Database Links

Gene Symbol

MAGEA4

UniProt

P43358

RefSeq (Protein):NP_001011550