MAGEA4 Antibody (N-term)

• Catalog Number: orb1930212
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2196
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 34899 Da
• Target: This MAGEA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human MAGEA4.
• UniProt ID: P43358
• NCBI: NP_001011549.1, NP_001011550.1, NP_001011548.1, NP_002353.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Melanoma-associated antigen 4, Cancer/testis antig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

The anti-MAGE-A4 Pab is used in Western blot to detect MAGE-A4 in A375 cell lysate.

Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1: 50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X.