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LRRK2 Antibody
SKU: orb150604
Description
Research Area
Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, WB |
|---|---|
| Dilution Range | WB (1:1000) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | N138/6 (Formerly sold as S138-6) |
| Immunogen | Fusion protein, amino acids 1-500 (N-terminus) of human LRRK2. 83% identical in mouse and rat. No significant identity with LRRK1. |
| Target | LRRK2 |
| Molecular Weight | 200kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−LRRK2, Leucine-rich repeat kinase 2, Leucine-rich repeat serine/threonine-protein kinase 2, RIP7, RIPK7, PARK8, Dardarin, ROCO 2, Augmented in rheumatoid arthritis 17
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone N138/6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_940980 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
LRRK2 Antibody (orb150604)
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