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Catalog Number | orb1247301 |
---|---|
Category | Antibodies |
Description | LRRK2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: CELAEKMRRTSV |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Strong expression of the protein seen in the vesicles of A431 cells and in the vesicles and nuclei of A549 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | LRRK2 |
UniProt ID | Q5S007 |
NCBI | NP_940980.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | LRRK2, PARK8, ROCO2, FLJ45829, DKFZp434H2111, leuc Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Strong expression of the protein seen in the vesicles of A431 cells and in the vesicles and nuclei of A549 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247301 (1.5 ug/ml) staining of paraffin embedded Human Hippocampus CA4. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
orb1247301 (2 ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
orb1247301 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody. This data is from a previous batch, not on sale.
orb1247301 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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