LRRK2 Antibody

SKU: orb1247301

Description

LRRK2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Strong expression of the protein seen in the vesicles of A431 cells and in the vesicles and nuclei of A549 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CELAEKMRRTSV
• Target: LRRK2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRRK2, PARK8, ROCO2, FLJ45829, DKFZp434H2111, leucine-rich repeat kinase 2, dardarin, Parkinson disease (autosomal dominant) 8, AURA17, DARDARIN, RIPK7, augmented in rheumatoid arthritis 17

orb1247301 (1.5 ug/ml) staining of paraffin embedded Human Hippocampus CA4. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1247301 (2 ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1247301 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody. This data is from a previous batch, not on sale.

orb1247301 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

LRRK2

UniProt

Q5S007

RefSeq (Protein):NP_940980.3