LRP4 Antibody

SKU: orb99087

Description

Mouse Anti-Mouse LRP4 (Extracellular) Monoclonal IgG2A

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-418 was sufficient for detection of LRP4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone No.: N207/27 (Formerly sold as S207-27)
• Immunogen: Fusion protein amino acids 26- 350 (extracellular N-terminus) of mouse LTP4
• Target: LRP4
• Molecular Weight: 215kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRP4, LRP-4, LRP10, MEGF7, Low density lipoprotein receptor related protein 4, Multiple epidermal growth factor like domains 7, LDLR dan, LDLR, KIAA0816, Corin

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone N207/27. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRP4 (Extracellular) Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 Monoclonal Antibody, Clone N207/27. Tissue: COS cells transfected with 2 ug human LRP4 plasmid. Species: Human. Fixation: 4% PFA for 10 min. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse: Alexa 488. Counterstain: DAPI.

Western Blot analysis of Rat brain membrane lysate showing detection of LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone N207/27. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:500, 1:1000, and 1:2000.

Quick Database Links

Gene Symbol

LRP4

UniProt

Q8VI56

NCBI Gene ID

83469

RefSeq (Protein):NP_766256.3