LRP4 Antibody (Biotin)

SKU: orb150133

Description

Mouse monoclonal to LRP4 (Biotin). Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. It is involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway..

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-418 was sufficient for detection of LRP4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone No.: N207/27 (Formerly sold as S207-27)
• Immunogen: Fusion protein amino acids 26- 350 (extracellular N-terminus) of mouse LTP4
• Target: LRP4
• Molecular Weight: 215kDa
• Purification: Protein G Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

LRP4, LRP-4, LRP10, MEGF7, Low density lipoprotein receptor related protein 4, Multiple epidermal growth factor like domains 7, LDLR dan, LDLR, KIAA0816, Corin

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone N207/27. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRP4 (Extracellular) Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 Monoclonal Antibody, Clone N207/27. Tissue: COS cells transfected with 2 ug human LRP4 plasmid. Species: Human. Fixation: 4% PFA for 10 min. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse: Alexa 488. Counterstain: DAPI.

Western Blot analysis of Rat brain membrane lysate showing detection of LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone N207/27. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:500, 1:1000, and 1:2000.

Quick Database Links

Gene Symbol

LRP4

UniProt

Q8VI56

NCBI Gene ID

83469

RefSeq (Protein):NP_766256.3