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LMX1B Antibody

SKU: orb1249582

Description

LMX1B Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 100kDa band in Mouse Kidney lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6kDa according to Human NP_002307.1 and to Mouse NP_034855.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 2.5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: QQALTTSCYFRDRK
TargetLMX1B
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

LMX1B, LIM homeobox transcription factor 1, beta, LMX1.2, MGC138325, MGC142051, NPS1, LIM homeo box transcription factor 1, beta

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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