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Catalog Number | orb2540996 |
---|---|
Category | Antibodies |
Description | LMX1b Rabbit Polyclonal Antibody (BF555) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Bovine, Canine, Human, Porcine, Sheep |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human LMX1b/NPS1 (111-210/379aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:100-500 |
Conjugation | BF555 |
Target | LMX1B |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | LIM homeo box transcription factor 1 beta; LIM hom Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-LMX1b Antibody, conjugated (orb2540996) at 1:400 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-LMX1b Antibody, conjugated (orb2540996) at 1:400 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.