LMX1A Antibody

SKU: orb1239578

Description

LMX1A Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus
• Application Notes:
  WB: 1-2 μg/mL; ICC/IF: 10-20 μg/mL; IHC-P: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rabbit polyclonal LMX1A antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A.The immunogen is located within amino acids 300 - 350 of LMX1A.
• Target: LMX1A
• Molecular Weight: Predicted: 42kD Observed: 45 kD
• Purification: LMX1A Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: LMX1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LMX1A Antibody: LMX1, LMX1.1, LIM homeobox transcription factor 1-alpha

Immunofluorescence Validation of LMX1A in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with orb1239578 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Western Blot Validation in Human Skeletal Muscle. Loading: 10 µg of lysates per lane. Antibodies: LMX1A, orb1239578, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Skeletal Muscle. Loading: 10 µg of lysates per lane. Antibodies: LMX1A, orb1239578, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Skeletal Muscle. Loading: 10 µg of lysates per lane. Antibodies: LMX1A, orb1239578, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of LMX1A in Human Brain Meningioma. Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody (orb1239578) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody (orb1239578) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody (orb1239578) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

LMX1A

UniProt

Q8TE12

RefSeq (Protein):NP_796372