LMW E.Coli Host Cell Protein Antibody

SKU: orb750541

Description

LMW E.Coli Host Cell Protein antibody

Images & Validation

• Tested Applications: 2D-PAGE, ELISA, WB
• Dilution Range: ELISA: 1:1000 - 1:2000, WB: 1:500 - 1:1000
• Reactivity: E. coli
• Application Notes:
  Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D, western blotting, and ELISA.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).
• Purity: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against low molecular weight E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store LMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 72.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, low molecular weight E.Coli HCP proteins antibody, LMW HCP antibody, 2D detection antibody

2D Western Blot of anti- E.coli Low Molecular Weight Host Cell Protein antibody. Load: 35 µg LMW HCP. Primary antibody: Rabbit anti-LMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.

ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein antibody. Antigen: BSA conjugates of LMW HCP. Coating amount: 1 µg per well titrated 1:3 in 1XPBS. Primary antibody: Anti-LMW-HCP antibody at 100 ng/well. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000. Substrate: TMB for 30 min.