LMW E.Coli Host Cell Protein Antibody

SKU: orb344698

Description

LMW E.Coli Host Cell Protein antibody

Images & Validation

• Tested Applications: 2D-PAGE, ELISA, WB
• Dilution Range: ELISA: 1:1000 - 1:2000, WB: 1:500-1:1000
• Reactivity: E. coli
• Application Notes:
  Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).
• Purity: LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.29 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-E.Coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-Low molecular Weight E.Coli Host Cell Proteins Antibody, Anti-Host Cell Protein, Low molecular weight E.Coli HCP proteins antibody

2D Western Blot of anti-E.coli Low Molecular Weight Host Cell Protein antibody. Load: 35 µg LMW HCP. Primary antibody: Rabbit anti-LMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.

ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein antibody. Antigen: BSA conjugates of LMW HCP. Coating amount: 1 µg per well titrated 1:3 in 1XPBS. Primary antibody: Anti-LMW-HCP antibody at 100 ng/well. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000. Substrate: TMB for 30 min.