LKB1 Rabbit pAb Discontinued

SKU: orb48550

This product can be custom made

Description

Rabbit polyclonal antibody to STK11

Research Area

Apoptotic, Autophagic, Cancer, DNA Damage, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 7

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human LKB1 (NP_000446.1).
Molecular Weight	49-60kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti LKB1 antibody, anti PJS antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunofluorescence analysis of NIH/3T3 cells using LKB1 Rabbit pAb (orb48550) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using LKB1 Rabbit pAb (orb48550) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using LKB1 Rabbit pAb (orb48550) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using LKB1 Rabbit pAb (orb48550) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of lysates from Mouse heart, using LKB1 Rabbit pAb (orb48550) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of various lysates, using [KO Validated] LKB1 Rabbit pAb (orb48550) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western blot analysis of various lysates, using LKB1 Rabbit pAb (orb48550) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.