STK11 (LKB1) Antibody (N-term I29)

SKU: orb1929449

Description

Research Area

Cancer Biology, Cell Biology, Metabolism Research

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	IHC-P - 1:100-500, WB - 1:1000, FC - 1:10-50, IF - 1:10-50
Reactivity	Human
Predicted Reactivity	Gallus, Mouse, Rat, Xenopus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	48636 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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LKB1, PJS

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

STK11 (LKB1) Antibody (N-term I29) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of STK11 (LKB1) Antibody (N-term I29) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

STK11 Antibody (I29) western blot analysis in T47D cell line lysates (35 ug/lane). This demonstrates the STK11 antibody detected the STK11 protein (arrow).

Western blot analysis of STK11 (arrow) using rabbit polyclonal STK11 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2).