LIGHT Antibody [7B9E12]

• Catalog Number: orb1239554
• Category: Antibodies
• Description: LIGHT Antibody [7B9E12]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7B9E12
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: LIGHT antibody was raised against the extracellular domain of human LIGHT.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26 kDa
• Target: TNFSF14
• UniProt ID: O43557
• NCBI: NP_003798
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: LIGHT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: LIGHT Antibody: TNFSF14, LTg, CD258, HVEML, HVEM l
• Note: For research use only
• Application notes: LIGHT antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μg/mL. LIGHT antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μg/ml

Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 μg/mL. Green: LIGHT Antibody [7B9E12] (orb1239554) Blue: DAPI staining

Immunofluorescence of LIGHT in human lymphoma tissue with LIGHT antibody at 20 μg/mL. Green: LIGHT Antibody [7B9E12] (orb1239554) Blue: DAPI staining

Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 μg/mL.

Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.