Goat anti-LIGHT / CD258 Antibody

SKU: orb20341

Description

Goat polyclonal to LIGHT

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Application Notes:
  This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 22kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 22.4kDa according to NP_742011.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LIGHT / CD258
• Protein Sequence: HEVNPAAHLTGANS
• Molecular Weight: 26.4; 22.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TNFSF14 antibody, anti tumor necrosis factor (ligand) superfamily, member 14 antibody, anti CD258 antibody, anti HVEML antibody, anti LIGHT antibody, anti LTg antibody, anti TR2 antibody, anti delta transmembrane LIGHT antibody, anti herpesvirus entry mediator A antibody, anti ligand for herpesvirus entry mediator antibody, anti tumor necrosis factor ligand superfamily, member 14 antibody, anti tumor necrosis factor receptor-like 2 antibody, anti tumor necrosis factor superfamily member LIGHT antibody

orb20341 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of Human Cerebellum (A) and Frontal Cortex (B) lysate using LIGHT antibody

Immunofluorescence analysis of HeLa cells using LIGHT antibody

Quick Database Links

Gene Symbol

LIGHT / CD258

NCBI Gene ID

8740