LIF Antibody

• Catalog Number: orb1538901
• Category: Antibodies
• Description: LIF Antibody
• Target: LIF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 16 AA synthetic peptide near the center of human LIF. The immunogen is located within aa 50-100 of LIF.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Application notes: Further information: LIF antibody can be used for detection of LIF by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunofluorescence starting at 20 ug/mL. Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 22 kDa Observed: 25 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LIF, DIA, Emfilermin, HILDA, Melanoma-derived LPL
• Note: For research use only

Immunofluorescence of LIF in 3T3 cells with LIF antibody at 20 ug/ml.

Western blot analysis of LIF in 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/ml.