LIF Antibody

• Catalog Number: orb1239553
• Category: Antibodies
• Description: LIF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-LIF antibody (orb1239553) was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 22kDObserved: 26kD (Post-modifications: N-linked Glycosylations)
• Target: LIF
• UniProt ID: P15018
• NCBI: NP_002300
• Storage: LIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: LIF Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inh
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse 3T3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL, 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Lung. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of LIF in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with orb1239553 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).