LIF (aa28-39) Antibody

• Catalog Number: orb167688
• Category: Antibodies
• Description: Goat polyclonal antibody to LIF
• Target: LIF (aa28-39)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TPVNATCAIRHP
• MW: 22
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.5-1.5 μg/ml
• Application notes: WB: Approx 23kDa band observed in Mouse and Rat Colon lysates (calculated MW of 22.3kDa according to Mouse NP_032527.1 and 22.1kDa according to Rat NP_071532.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CDF antibody, anti cholinergic differentiatio
• Note: For research use only
• Entrez: 3976
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse Colon lysate (35ug protein in RIPA buffer) using LIF antibody

Western blot analysis of Mouse(lane 1) and Rat(lane 2) Colon lysate using LIF antibody