LEP Antibody (N-term)

SKU: orb164246

Description

Rabbit polyclonal antibody to Leptin

Research Area

Cardiovascular Research, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50, FACS: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB36463
• Immunogen: Synthetic Peptide
• Target: LEP
• Molecular Weight: 18641
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

anti LEP antibody, anti LEPD antibody, anti Leptin antibody, anti Leptin Murine Obesity Homolog antibody, anti Leptin Precursor Obesity Factor antibody, anti OB antibody, anti Obese Protein antibody, anti Obese, mouse, homolog of antibody, anti Obesity antibody, anti Obesity factor antibody, anti Obesity factor antibody, anti Obesity homolog mouse antibody, anti Obesity Murine Homolog Leptin antibody, anti OBS antibody

LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the LEP antibody detected the LEP protein (arrow).

Confocal immunofluorescent analysis of LEP Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.

LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

LEP

UniProt

P41159

RefSeq (Protein):NP_000221.1