Leptin Receptor (LEPR) Antibody (N-term)

• Catalog Number: orb1930224
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Leptin Receptor (LEPR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human Leptin Receptor (LEPR).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P48357
• MW: 132494 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB02053
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leptin receptor, LEP-R, HuB219, OB receptor, OB-R,
• Note: For research use only
• NCBI: NP_001185618.1, NP_001185616.1, NP_001003680.1, NP_001003679.1, NP_002294.2, NP_001185617.1

Leptin Receptor (LEPR) Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the LEPR antibody detected the LEPR protein (arrow).