Lanabecestat

SKU: orb1679015

Description

Lanabecestat

Research Area

Neuroscience

Key Properties

• CAS Number: 1383982-64-6
• MW: 412.53
• Purity: 98.55%
• Formula: C₂₆H₂₈N₄O
• SMILES: COC1CCC2(Cc3ccc(cc3[C@@]22N=C(C)C(N)=N2)-c2cncc(c2)C#CC)CC1
• Target: Beta-Secretase,BACE
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (8 mM);DMSO:80 mg/mL (193.93 mM)

Bioactivity

• Target IC50:
  BACE1:0.4 nM (cell free)|BACE1:0.4 nM
• In Vivo:
  Lanabecestat treatment (1 mg/kg; oral gavage; once daily; for 3 weeks) increased the bursting frequency in 4-AP-induced cell models in Bace1-null mice.
• In Vitro:
  Lanabecestat differentially altered the protein levels of microglia in 5xFAD and APP KI mice.
• Cell Research:
  Cells are incubated with different Lanabecestat concentrations for 5 to 16 h, and the release of sAβPPβ, Aβ1-40, Aβ1-42, or sAβPPα into the medium is analyzed using kits. Cytotoxic effect of Lanabecestat is evaluated in the cell plates using cell proliferation/cytotoxicity kit.
• Animal Research:
  Female 7- to 14-week-old C57BL/6 mice (n=6 per treatment group and time point) receive a vehicle or Lanabecestat solution at 50, 100, or 200 μmol/kg (20, 41, or 82 mg/kg) as a single dose via oral gavage. Mice and guinea pigs are anesthetized 1.5, 2, 3, 4, 6, 8, 16, 24, or 48 h after the (last) administration of vehicle or drug and are then kept under isoflurane anesthesia. Cerebrospinal fluid (CSF) is aspirated from the cisterna magna, and plasma is isolated from blood collected by cardiac puncture into EDTA tubes. The animals are then sacrificed by decapitation, and the brains are dissected into hemispheres.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AZD3293, AZD-3293, AZD 3293, BACE1, Lanabecestat, LY 3314814, LY3314814, LY-3314814

Compound Identifiers

PubChem CID

67979346