L1CAM Antibody (C-term)

SKU: orb1935060

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P - 1-600, WB - 1:8000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This L1CAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1154-1182 amino acids from the C-terminal region of human L1CAM. Antigen Region: 1154-1182 aa.
• Target: L1CAM
• Molecular Weight: 140003 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, CD171, L1CAM, CAML1, MIC5

L1CAM Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the L1CAM antibody detected the L1CAM protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. Diluted at 1~600 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-L1CAM Antibody (C-term) at 1: 8000 dilution + human fetal brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

L1CAM

UniProt

P32004

RefSeq (Protein):NP_001137435.1, NP_001265045.1, NP_076493.1, NP_000416.1