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Anti-L1CAM Antibody

Catalog Number: orb315797

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb315797
CategoryAntibodies
DescriptionRabbit polyclonal antibody to L1CAM
TargetL1CAM
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat, Zebrafish
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human L1CAM. The exact sequence is proprietary.
UniProt IDP32004, Q05695, P11627
Tested applicationsIF, IH, WB
Dilution rangeWB: 1-500:1000, IF/ICC: 1-100:500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti CAML1 antibody, anti MIC5 antibody, anti Neur
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NoteFor research use only
Entrez3897, 50687
Anti-L1CAM Antibody

Western blot analysis of L1CAM expression in HEK293T (A), A549 (B) whole cell lysates. (Predicted band size: 140 kD; Observed band size: 140 kD)

Anti-L1CAM Antibody

Immunohistochemical analysis of L1CAM staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-L1CAM Antibody

Immunofluorescent analysis of L1CAM staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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