ansB Antibody (HRP)

SKU: orb344902

Description

L-Asparaginase antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1,000
• Reactivity: E. coli
• Application Notes:
  Anti-Asparaginase Peroxidase conjugated antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and by ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
• Target: ansB
• Purity: Anti-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-L-Asparaginase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody. Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.

Quick Database Links

Gene Symbol

ansB

UniProt

P00805

RefSeq:P00805.2